Deterministic genomic screening for bacteriophage therapeutics
Automated multi-tool genomic audit evaluating contig completeness, strictly virulent lytic compliance, absence of antimicrobial resistance, non-toxigenic virulome profiling, and target bacterial host interaction — producing publication-ready clinical dossiers.
NC_019713 Vibrio phage vB_VpaS_MAR10
Cleared Quality Gate: High-quality phage genome exceeding 90.0% completeness requirement with 0% host chromosomal contamination.
Identified complete structural modules, DNA replication machinery, and an intact dual-component lysis cassette (holin, endolysin, spanin operons).
ProstT5 deep structural protein language embeddings resolved previously uncharacterized tail fibers and receptor-binding domains.
Zero antimicrobial resistance genes or antibiotic-inactivating enzyme determinants detected against Comprehensive Antibiotic Resistance Database.
Zero bacterial exotoxins, enterotoxins, superantigens, or pathogenic virulence factors found across all predicted coding sequences.
Absence of anti-CRISPR genetic elements, confirming candidate remains CRISPR-sensitive with low defense-suppression risk.
Random forest classification confirmed strictly virulent lytic lifecycle; zero integrase or site-specific recombinase genes detected.
Deep protein-protein embedding predicted high-affinity binding to target bacterial pathogen with 95.0% confidence.
Monophyletic cluster analysis assigned candidate to Caudoviricetes; Straboviridae; Tequatrovirus with robust proteomic overlap.
Screening modules & safety checkpoints
CheckV Contig Screening
Verifies complete circular terminal repeats, assesses host contamination percentage, and ensures candidate length satisfies the therapeutic 20–700 kb window.
pharokka & phold (ProstT5)
Combines fast HMM gene prediction with 3D structural protein language models to resolve unknown hypothetical proteins, baseplates, and tail fibers.
CARD, RGI, VFDB & AcrFinder
Enforces zero-tolerance safety screening for transmissible antibiotic resistance determinants, bacterial exotoxins, and anti-CRISPR genetic elements.
BACPHLIP & PHIEmbed
Classifies virulent lytic vs temperate lysogenic lifestyles, checks for absence of integrases/recombinases, and predicts high-affinity bacterial host targets.
Ready to evaluate candidate phage genomes?
Upload raw FASTA sequences to receive full 10-tool biological audits and downloadable regulatory evidence dossiers.
🚀 Open Astra-Ya Beta Console